| 摘要: |
| 以羔羊皱胃为原料，研究了硫酸铵和食盐盐析法纯化羔羊皱胃酶的工艺条件，系统分析了食盐饱和度、pH、盐析温度以及盐析时间对羔羊皱胃酶纯化效果的影响。结果表明，硫酸铵不适宜用于盐析羔羊皱胃酶,而食盐是羔羊皱胃酶适宜的盐析剂。食盐盐析羔羊皱胃酶的适宜盐析条件为：食盐饱和度55%～65%，pH 4.00～4.60，盐析温度4 ℃，盐析时间16 h。 |
| 关键词: 羔羊皱胃酶 盐析条件 酶纯化 |
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| 基金项目:陕西省自然科学基金资助项目（2003Ci34） |
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| Study on the condition for salting-out of goat kid rennet |
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| Abstract: |
| Using goat kid abomasums as material,the purification conditions of goat kid rennet by salting out with (NH4)2SO4 and NaCl were investigated.The effects of salt saturation,salting-out pH,temperature,and salting-out time on the purification efficiency were systematically analyzed.The results showed that ammonium sulfate was proved not to be suitable to precipitate rennet.But NaCl could be an optimum salting-out agent in the salting-out of goat kid rennet.The optimal condition for salting-out by NaCl was 55%-65% NaCl saturation,salting-out pH 4.00-4.60,temperature 4 ℃ and salting out time 16 h. |
| Key words: goat kid rennet salting-out method purification |
