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响应曲面法优化苦荞麸皮总黄酮的提取工艺
王 军1, 王 敏1, 宋旭辉2
1.西北农林科技大学 食品科学与工程学院;2.扶风县上宋中学
摘要:
测定了苦荞麸皮总黄酮的含量,并采用响应曲面法中的Box-Behnken模式,对苦荞麸皮总黄酮微波辅助提取工艺进行了优化。结果表明,苦荞麸皮总黄酮含量为60.1 g/kg;其提取的最佳工艺条件为微波加热时间120 s,乙醇体积分数86%,料液比1∶50,在此条件下总黄酮得率达58.1 g/kg,提取率达96.67%。
关键词:  苦荞麸皮  总黄酮  微波  响应曲面法  提取工艺
DOI:
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基金项目:科技部攻关计划重大项目(2003BA901A19);西安市2003年农业科技攻关计划项目(NG200317);西北农林科技大学人才基金项目;2005年西北农林科技大学科研基金项目
Study on extraction technology of total flavonoids in tartary buckwheat bran using response surface methodology
Abstract:
In this paper,the content of total flavonoids in tartary buckwheat bran was determined,the extraction technology of total flavonoids in the bran by microwave was studied,and all experiments were designed and optimized with Box-Behnken concept of response surface methodology (RSM).The results showed that the content of total flavonoids was 60.1 g/kg;The regression equation (model) for the extraction technology was established,and also verified with the check experiments.The optimum conditions of the extraction technology were obtained as follows:treatment time 120 s,the concentration of ethanol 86%,the ratio of material to solvent 1∶50.Using the technological conditions,the extraction yield of total flavonoids was 58.1 g/kg,and the extraction rate was 96.67%.
Key words:  tartary buckwheat bran  total flavonoids  microwave  response surface methodology (RSM)  extraction technology