| 摘要: |
| 以吉林小粒1号大豆为材料进行卡那霉素梯度涂抹试验,确定卡那霉素筛选的最佳浓度和筛选临界观察时间,并在此基础上,对转基因大豆植株(含nptⅡ基因)分离后代进行卡那霉素涂抹检测和PCR检测,检验2种方法的符合度。结果表明,卡那霉素的最佳筛选浓度为500 mg/L,观察时间以3 d为宜;抗和高抗卡那霉素植株2种检测方法的符合度为90.8%,感和高感卡那霉素植株的符合度为87.1%。可见,卡那霉素叶片涂抹法完全可以用于大豆转基因植株的快速筛选。 |
| 关键词: 大豆 转基因植株 卡那霉素 |
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| 基金项目:国家“973”项目(2002CB111301-3) |
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| Effectiveness of screening the transformed plants by smearing Kanamycin on the soybean leaves |
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| Abstract: |
| Kanamycin can be used to select the transformed plants containing the nptⅡ gene.In order to assay the optimal screening concentration to nptⅡ gene,Kanamycin was smeared to the surface of the transformed soybean leaves of Jilinxiaoli No.1.It showed that 500 mg/L Km with 3 days electing was best.This method was compared with PCR as well,and the results suggested that the equivalent ratio was 90.8% between the high-resistant plants and the resistant plants,87.1% between the sensitive plants and the hypersensitive plants.Accordingly,it is available to select the transformed soybean plants by smearing with Km. |
| Key words: soybean gene-transformed plant polymerase chain reaction Kanamycin |
