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养殖水体高效氨氮脱除菌的分离及脱除特性研究
侯 颖1,2, 徐建强3, 孙军德2
1.河南科技大学 食品与生物工程学院;2.沈阳农业大学 土地与环境学院;3.河南科技大学 林学院
摘要:
利用以(NH4)2SO4为惟一氮源的选择性培养基,从养鱼池塘水中分离筛选到1株高效氨氮脱除菌X2,当氨氮初始质量浓度为50 mg/L时,该菌株在24 h内的氨氮脱除率可达95%以上。初步鉴定该菌株为巨大芽孢杆菌(Bacillus megaterium),同时该菌株还具有硝酸和亚硝酸还原能力。对X2菌株的氨氮脱除特性进行初步研究结果表明,该菌株的生长与氨氮脱除同步进行;其脱除氨氮的最适温度和pH值分别为30℃和7.0;当氨氮初始质量浓度在50mg/L以下时,X2菌株基本可将培养基中的氨氮完全脱除。
关键词:  养殖水体  氨氮脱除菌  巨大芽孢杆菌  氨氮脱除率
DOI:
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Studies of isolation and removement characteristics of high effective microorganisms with ammonia-nitrogen remaved in aquatic water
Abstract:
A highly effective microorganism with the removal of ammonia nitrogen X2 has been isolated and screened from a fishpond by the selected culture with (NH4)2SO4 as exclusive nitrogen source.The removing rate of ammonia nitrogen of X2 is over 95% within 24 h when the beginning mass concentration of ammonia nitrogen is 50 mg/L.X2 strain is identified Bacillus megaterium primarily and it can reduce nitrate and nitrite simultaneously.The removement characteristics of X2 have been studied,and the results show that:the growth of X2 strain and the removement of ammonia nitrogen are synchronous;the optimum temperature and pH of X2 strain with ammonia nitrogen removed are 30 ℃ and 7.0 respectively;ammonia nitrogen of culture medium can be removed completely when the initial mass concentration of ammonia nitrogen is under 50 mg/L.
Key words:  aquatic water  microorganisms with ammonia-nitrogen removed  Bacillus megaterium  removing rate of ammonia-nitrogen