| 摘要: |
| 利用PCR反应延伸过程中,3′端碱基不配对,PCR反应不能启动的等位基因特异性PCR分析法(AS-PCR)原理,采用生物软件DNASTAR分析牛、羊线粒体细胞色素b(Cytb)基因,以牛、羊碱基差异较大位点作为上游引物的3′端来设计引物,进行PCR扩增反应,分析牛羊异种克隆胚中线粒体的变化情况。结果表明,AS-PCR法是一种快速、有效鉴定异种重构胚中线粒体异质性的方法。 |
| 关键词: 核移植 线粒体 细胞色素b |
| DOI： |
| 分类号: |
| 基金项目:国家“863”高科技项目（2001AA213081） |
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| Application of AS-PCR in interspecies transplantation between goat and cattle |
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| Abstract: |
| In the PCR elongation process,the reaction can’t start when 3′-end base doesn’t match.To study the heteroplasmy of mitochondria,bio-assay software DNASTAR was used to align Cytb gene of cattle and goat.Then PCR primers were designed with different bases between goat and cattle with 3′-end base as upstream primers.The change of mitochondria in interspecies goat-cattle nuclear transfered embryos was identified by AS-PCR.The results indicate that AS-PCR is a rapid and efficient method to identificate mitochondria heteroplasmy in interspecies reconstructed embryos. |
| Key words: AS-PCR interspecies nuclear transfer mitochondria cytochrome b |
