| 摘要: |
| 采用基因敲除技术，作工业酿酒酵母染色体DNA上的甘油代谢途径关键酶基因GPD1中，整合入来源于里氏木霉的β-葡萄糖苷酶基因荩bglⅡ．通过提高G418浓度筛选得到多拷贝整合子。结果表明，融合子利用纤维二精的能力得到提高，产甘油能力下降，外源基闪表达稳定；引入外源基因后．其对酵母增殖能力没有大的影响，仅形态发生变化；在以微晶纤维素为原料，结合纤维素酶发酵时，与亲代工业酿酒酵母相比，发酵液乙醇浓度最高增加69％。 |
| 关键词: β-葡萄糖苷酶 纤维二糖 纤维素利用 酒精发酵 |
| DOI： |
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| 基金项目:国家科技部“十五”攻关项目（2001BA501A01） |
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| Ethanol fermentation from cellulose by expression of cellobiase gene and disruption of GPD1 in Saccharomyces cerevisiae |
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| Abstract: |
| According to homologous recombination theory,β-glucosidase gene bglⅡ from Trichoderma reesei was integrated into the key enzyme gene GDP1 of the glycerol metabolism pathway from industrial Saccharomyces cerevisiae chromosome DNA.And multi-copy recombinants were screened through increasing G418 concentration in the medium.It was shown,based on the study,that cellobiose utility capability of the recombinants was increased,glycerol productivity of that was decreased,and the bglⅡ gene expression was stable in the host cell.No impact on the cell character appeared after the introduction of extrinsic gene,but the cells fluctuated when growing.When compared with parent industrial S.cerevisiae Y to ferment with microcrystalline cellulose for raw material incorporation cellulase,ethanol strength increased by 69% and cellobiose accumulation decreased. |
| Key words: β-glucosidase cellobiose cellulose utility ethanol fermentation |
