| 摘要: |
| 研究了不同分离培养基及样品预处理（热处理和化学处理）对盐碱土中放线菌分离效果的影响。结果表明：（1）碱土中放线菌的总数、属数和链霉菌类群数极显著地高于盐土；（2）高氏1号琼脂为最佳的基础培养堆，精氨酸-甘油琼脂次之；（3）A3培养基（高氏1号琼脂。NaCl 0.5 g/kg，pH9.0）为碱土的最佳分离培养基；A1培养基（高氏1号琼脂，NaCl 50 g/kg，pH7.0）为盐土的最佳分离培养基；（4）盐碱土用无菌的20 g/kg腐殖酸溶液浸泡，于40 ℃下振荡20 min。町提高放线菌的出菌率并抑制杂菌生长。 |
| 关键词: 盐碱土 放线菌 分离培养基 样品预处理 出菌率 |
| DOI： |
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| 基金项目:西北农林科技大学土壤学博士点基金项目（2003） |
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| Study on the isolation methods of actinomycetes from saline-alkaline soils |
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| Abstract: |
| The effect of basic culture media,saline content,pH and different pretreatments of saline-alkaline soils on actinomycete isolation was studied.The results showed that the quantity of actinomycetes,genus and streptomyces varieties in alkaline soils was higher than that in saline soils;Gaoshi No.1 agar was best among all the basic culture media,next was Arginine-Glycerine agar;The basic culture media holding the moderate pH(pH 9.0) and saline concentration (NaCl 50 g/kg) was good to actinomycete isolation;A3 (NaCl 0.5 g/kg，pH 9.0) was the best isolation culture media when applying for alkaline soils;A1 (NaCl 50 g/kg,pH 7.0) was the best isolation culture media when applying for saline soils;It could promote actinomycete development and inhibit the development of other microorganism when the saline alkaline soil was immersed in HA 20 g/kg solution and vibrated at 40 ℃ for 20 minutes. |
| Key words: saline alkaline soil actinomycete isolation culture media pretreatrnent microbe ratio isolation |
