| 摘要: |
| 以水稻土浸提液为接种液、人工合成的无定形氧化铁为唯一的电子受体、添加的苯系物（甲苯、苯胺、苯酚、苯甲酸）为唯一碳源，在适量无机N，P，K营养供给条件下，采用厌氧恒温混合培养方法，研究了厌氧微生物对氧化铁还原的影响。结果表明，由水稻土中提取的微生物可以利用苯系物作为唯一碳源，在厌氧降解的同时将电子转移给Fe（Ⅲ），使其还原为Fe（Ⅱ）；利用不同苯系物的铁还原过程难易程度有所差异，铁还原率表现为苯甲酸最高（53.99％），甲苯最低（19.55％），利用苯系物的铁还原率均低于以葡萄糖为碳源时的铁还原率（71.16％）；不同浓度苯系物对氧化铁还原过程有不同程度的影响，在一定浓度范围内，随着苯系物浓度的增加其对氧化铁还原过程具有促进作用，而高浓度苯系物则对氧化铁还原过程表现出抑制趋势；以2．0 mmol/L苯系物作为碳源时，蒽醌-2，6二磺酸盐（AQDS）可明显增加Fe（Ⅱ）的累积量。 |
| 关键词: 异化铁还原 苯系物 水稻土 蒽醌-2 6-二磺酸盐 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目（40271067）；教育部博士学科点专项科研基金项目（20020712009） |
|
| Effects of benzene analogs as sole carbon source on dissimilatory iron reduction |
|
|
| Abstract: |
| The paddy soil extraction was incubated anaerobically as inoculum and dissimilatory iron reduction was investigated during anaerobic co-culture containing ferrihydrite as sole electron acceptor,benzene analogs (toluene,aniline,phenol,benzoic acid) as sole carbon source,under suitable supplies of inorganic N,P and K nutrition.The results indicated that,the microbe in the paddy extraction could transfer electrons to Fe(Ⅲ),utilizing benzene analogs as sole carbon source when microbe was degraded;the iron reduction rates were different,the maximal reduction was 53.99% with benzoic acid as carbon source and the minimum reduction was 19.55% with toluene as carbon source;the reduction level with benzene analogs as carbon source was less than that with glucose as carbon source (71.16%);different concentrations of benzene analogs had different effects on iron reduction;under suitable concentration range of benzene analogs,dissimilatory iron reduction depending upon the microorganism activities could be strengthened,however,there was a trend that iron reduction was to be suppressed when the concentration of benzene analogs was too high.The addition of anthraquione-2,6-disulfonate during co-culture could obviously increase Fe(Ⅱ) level with 2.0 mmol/L of benzene analogs as carbon source.This showed that anthraquione-2,6-disulfonate could promote the transference of electrons from the benzene analogs to ferrihydrite. |
| Key words: dissimilatory iron reduction benzene analogs paddy soil anthraquione-2,6-disulfonate |
