| 摘要: |
| 采用PCR方法对草鱼MyoD基因开放阅读框进行了改造扩增，构建了草鱼MyoD基因的原核表达载体pBV220-MyoD，并进行了初步的原核表达研究。结果表明，构建的原核表达载体pBV220-MyoD含有草鱼MyoD基因完整的开放阅读框；该原核表达载体表达产物的相对分子质量为34ku，其表达产物占全菌蛋白的10.8％，表达量较低。 |
| 关键词: 草鱼 MyoD基因 原核表达 |
| DOI： |
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| 基金项目:中国水产科学研究院重点基金项目（99-08-02） |
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| Expression of grass carp MyoD in Escherichia coli |
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| Abstract: |
| PCR was used to reconstruct the open reading frame of grass carp MyoD,and the expression vector pBV220-MyoD was constructed to research the expression of MyoD in E.coli on this basis.The results showd that:The pBV220-MyoD was constructed successfully,and there was a proper band at the 34 ku,after the expression vector was induced at 42 ℃,but the level of expression was not so high,only 10.8% of the total proteins of Escherichia coli. |
| Key words: grass carp MyoD gene expression in E.coli |
