| 摘要: |
| 研究了植酸（PA）对生防细菌L-227-03生长、发酵及抑菌能力的影响。结果表明：（1）在发酵6 h添加0.006％PA，18 h添加0.004％PA对L-227-03菌株细胞生长的促进作用最强，达到最佳的添加效果。（2）添加PA能提高L-227-03菌株细胞增殖速度，促进菌株提前6 h进入对数生长期，稳定期延长6 h。（3）添加PA发酵后，生防细菌L-227-03的抑菌活性增强，皿内抑菌圈直径最大达24 mm，较对照大6 mm，萝卜块根防效试验和盆栽防效试验的最高防效分别为82.34％和82.37％，较对照提高了13.39％和12.26％；菌体发酵液的耐保藏能力也有所提高，发酵液的有效期限延长3～6个月。（4）PA能缓和pH变化对菌体生长的影响，提高菌体对盐的耐受力。 |
| 关键词: 植酸 生防细菌 细菌生长 细菌发酵 抑菌能力 |
| DOI： |
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| 基金项目:西北农林科技大学科技创新团队项目 |
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| Effects of phytic acid on the growth,fermentation and antagonism of biocontrol bacterium L-227-03 |
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| Abstract: |
| The effects of Phytic Acid(PA) on the growth,fermentation and antagonism of biocontrol bacterium L-227-03 were studied.The results showed that:(1) The optimum addition of PA was adding(0.006%) after fermented 6 h and adding 0.004% after fermented 18 h,which best promote the cells growth of strain L-227-03.(2) PA accelerated cells proliferation rate,made exponential phase ahead of schedule for 6 h and stationary phase extended for 6 h.(3) After PA was added,the antagonism of L-227-03 was advanced,the biggest inhibiting zones in plate was 24 mm,bigger than CK by 6 mm,the strongest control effect of radish method and pot experiment were 82.34% and 82.37%,better than CK by 13.39% and(12.26%);After PA was added,fermented liquid ability of storage was improved and the time of fermented liquid preservation was prolonged by 3-6 months.(4) PA depressed the influence of pH and enhanced the salt-tolerance of this strain. |
| Key words: Phytic Acid biocontrol bacterium growth fermentation antagonism |
