| 摘要: |
| 用RT-PCR方法对分离于陕西省不同地区的8个鸡肾型IBV毒株的N基因分别进行了扩增,将各扩增产物与T载体连接后,转化感受态细胞筛选阳性克隆质粒并进行基因测序,用DNAstar软件将这8个毒株与GenBank收录的7个IBV代表毒株的N基因进行了比较分析,研究了其变异情况.结果表明,B01,YL,WH和YX株与7株代表毒株的同源性较高,而G,BJ,WG,FF株与7个代表毒株的同源性相对较低.氨基酸推导序列比对结果表明,N蛋白突变以散在的点突变形式为主,其中46,48,50,75,78,189,232,236,237,299,301,350,366等位点容易发生突变,突变位点无明显规律性. |
| 关键词: 肾型传染性支气管炎病毒 地方分离株 N基因 序列分析 鸡 |
| DOI： |
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| 基金项目:陕西省科技攻关项目（2005K01-G20-02）；西北农林科技大学科研专项（20030203015） |
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| Clone and genetic variation analysis of N gene of nephropathogenic avian infectious bronchitis virus isolates |
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| Abstract: |
| The complete N genes of eight avian infectious bronchitis viruses (IBV) isolated from different places of Shaanxi province were amplified by using RT-PCR method.The products were ligated with Tvector separately and the transfered plasmids were obtained respectively.The N genes were sequenced in invitorgen cooperation.Sequences were analyzed among the eight isolates in China and the reference strains in GenBank by using DNAstar software.According to the analyzed results of N genes nucleotide, BO1 strain,YL strain,WH strain and YX strain share high identities,while the identities of G strain,BJ strain,WG strain and FF strain are low.The results of deduced amino acid sequences show that the mutation of N protein mainly display sporadic point mutation as 46,48,50,75,78,189,223,236,237,298,301,350,366 et al.Mutational site did not show obvious regularity. |
| Key words: nephrotropic avian infectious bronchitis virus local isolate strains N gene sequence analysis chicken |
