| 摘要: |
| 为了确定大鼠骨髓间充质干细胞（Mesenchymal stem cells,MSCs）体外分离和培养的方法,建立了稳定的MSCs体外培养扩增体系,通过密度梯度离心和贴壁筛选法分离培养大鼠MSCs,观察了细胞的形态,研究了其增殖及生长特征,并应用流式细胞术测定了细胞周期及CD29,CD44,CD45和HLA-DR的表达.结果表明,在体外培养条件下大鼠MSCs贴壁生长,为成纤维细胞样;CD45和HLA-DR的表达呈阴性,CD29和CD44的表达呈阳性;细胞周期的G0/G1期约占95%,具有原始细胞的特征.说明建立的体外培养扩增体系可获得形态单一、生长稳定、增殖性较强、较均一的大鼠MSCs. |
| 关键词: 大鼠 骨髓间充质干细胞 细胞形态 细胞周期 密度梯度离心 流式细胞术 |
| DOI： |
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| 基金项目:国家自然科学基金项目（39900036） |
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| Isolation and culture of rat marrow-derived mesenchymal stem cells in vitro |
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| Abstract: |
| To investigate the isolation and culture of mesenchymal stem cells (MSCs) in vitro from rat bone marrow,a steady culture system of MSCs for future study was established.The mononuclear cells derived from rat marrow were obtained from rat bone marrow by density gradient centrifugation and selected by cell attachment.The morphology of MSCs was observed by microscope.The cell cycle and surface antigens were identified by flow cytometry.The growth curve of cells was analysed through MTT experiment.The results showed that MSCs were fibroblast-like cells.They were positive for CD29 and CD44,but were negative for CD45 and HLA-DR.About 95% of MSCs were in G0/G1 phase and MSCs had some primordial cells characteristics.Morphology of MSCs was singula and their growing was stable.In addition,proliferation of MSCs was high. |
| Key words: rat bone marrow mesenchymal stem cells cell morphology cell cycle density gradient centrifuge separation flow cytometry |
