| 摘要: |
| 应用RT-PCR法对分离于不同禽类的IBDV HN01株（鸡源）、WJ株（乌鸡源）和YL997株（鸭源）的VP2基因分别进行了克隆，对其与核苷酸序列及其编码的VP2蛋白氨基酸序列进行了分析，并对此3株IBDV与标准血清Ⅰ型STC株的VP2基因和VP2蛋白进行了同源性分析。结果表明，WJ株、LY997株、HN01株与标准血清Ⅰ型STC株的核苷酸同源性分别为91.30％，92.50％和93.47％，氨基酸同源性分别为92.74％，91.90％和95.10％；HN01株与WJ株的核苷核和氨基酸的同源性分别为97.9％和98.4％。HN01株与LY997株的核苷酸和氨基酸的同源性分别为98.7％和99.05％，WJ株与LY997株的核苷酸和氨基酸同源性分别为99.1％和97.68％，3个毒株的VP2基因序列均完全具备超强毒株的主要特征，均为超强毒株，且与标准血清Ⅰ型STC株的核苷酸和氨基酸同源性均较低；3个野毒株之间虽具有较高的同源性，但相互之间也有一定的差异。 |
| 关键词: 鸡 乌鸡 鸭 传染性法氏囊病毒 VP2基因 克隆 序列分析 |
| DOI： |
| 分类号: |
| 基金项目:河南省科技攻关项目（0524030004） |
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| Cloning and sequence analysis of the VP2 gene of IBDV isolated from different fowls |
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| Abstract: |
| The VP2 gene of IBDV field strains HN01 (chicken),WJ (dark-bone chicken) and LY997 (ducks) were amplified by RT-PCR and cloned into PMD18-T vector to analyze sequence.The sequences of WJ,LY997 and HN01 isolates were compared with standard Ⅰ sterotype IBDV (STC).The homology of the nucleotide were 91.30%,92.50%,93.47%,and the homology of amino acids were only 92.74%,91.90%,95.10% separately;Between HN01 isolate and WJ isolate,the homology of the nucleotide and amino acids were 97.90% and 98.40% respectively;HN01 sequence was compared with LY997 isolate,the homology of the nucleotide and amino acids were only 98.7% and 99.05%;and the homologies of nucleotide and amino acids were only 99.1% and 97.68% between WJ isolate and LY997 isolate.The VP2 gene sequences of the three isolates all possessed the characteristics of very virulent IBDV (vvIBDV),the homologies of nucleotide and amino acids of the three isolates and standard Ⅰ stertype IBDV were very low.Although the homology of the three isolates were very high,there are some differences among them. |
| Key words: chicken,duck-bone chicken,duck,infection bursal disease virus(IBDV),VP2 gene,clone,sequence analysis |
