| 摘要: |
| 以4个马铃薯栽培品种为试材，进行了其茎段的离体再生研究。结果表明，东农303、夏波帝、鄂1号茎段愈伤组织最佳诱导培养基为MS＋6BA 3.0 mg/L＋NAA0.01 mg/L；费乌瑞它为MS＋6-BA 3.0 mg/L＋NAA 0.1 mg/L，愈伤组织的诱导率均可达100％。费乌瑞它和鄂1号不定芽分化诱导的最佳培养基均为MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L；东农303为MS＋6-BA 2.0 mg/L＋NAA 0.1 mg/L；夏波带为MS＋6-BA 2.0 mg/L＋NAA 0.3 mg/L，其不定芽分化率分别达96.7％，96.7％，83.3％和80.0％。各品种不定芽的诱导生根率均为100％，试管苗移栽成活率为95％。 |
| 关键词: 马铃薯 茎段 愈伤组织 植株再生 |
| DOI： |
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| 基金项目:中德政府间合作项目(GH-01/04);中德农业部科技合作项目 |
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| Study on in vitro regeneration technology from stem explants of four potato cultivars |
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| Abstract: |
| Four potato cultivars were regenerated in vitro from stem explants.The results showed that the optimal medium for callus induction was found to be MS+6-BA 3.0 mg/L+NAA 0.01 mg/L for cv.Dongnong 303,cv.Shepody and cv.E No.1,MS+6-BA 3.0 mg/L+NAA 0.1 mg/L for cv.Favorite.The callus induction rate on those media reached up to 100%.The optimal medium for adventitious bud differentiation from stem callus was found to he MS+6-BA 2.0 mg/L+NAA 0.2 mg/L for cv.Favorite and cv.E No.1,MS+6-BA 2.0 mg/L+NAA 0.1 mg/L for cv.Dongnong 303,and MS+6-BA 2.0 mg/L+NAA 0.3 mg/L for cv.Shepody.The adventitious hud differentiation rate reached 96.7%,96 7% 83.3% and 80.0% for the four cultivars respectively,and the rooting rate was 100% for all cultivars The survival rate of tube plantlets after transplantation reached 95%. |
| Key words: potato stem section callus plant regeneration |
