| 摘要: |
| 利用X精子在酸性环境条件下的运动活性比Y精子强的原理,通过酸诱导,用王氏管分离法(实时精子分离技术)分离家兔X、Y精子,同时利用体视显微镜评定所分离精子的活率,并用喹吖因染色法进行鉴定。结果表明,在稀释液pH为5．5时分离效果最好,分离后目的精液精子活率平均达(91±1.4)％,较分离前平均精子活率(82±1.3)％提高了9.1％,两者差异极显著(P<0.01);分离后的精液中,X精子的比例为(73.3±1.0)％,Y 精子的比例为(26.7±1.0)％,与分离前精液中X、Y精子各占50％的比例差异均极显著(P<0.01),表明酸诱导条件下的王氏管分离法是X、Y精子分离简单有效的方法。 |
| 关键词: 酸诱导 X、Y精子 王氏管分离法 BWW液 F小体 |
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| 基金项目:河北省秦皇岛科技局“转基因动物生产基因工程药物研究”项目（D08） |
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| The separation of X and Y spermatozoa in the environment of acid inducement |
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| Abstract: |
| Separating animal’s X and Y spermatozoa is the most direct and efficient way to control animals sex.Real-time separation technology was used in our study and its theory is that the X spermatozoa swims faster than Y spermatozoa in acidic solution,X spermatozoa are aim spermatozoa and the separation result（motility of separated sperm,rate of X spermatozoa）was identified with microscope and Quarine dyeing.In order to find the suitable pH value of separation solution,the original sperm was diluted with BWW solution in different pH values,finally,the pH 5.5 solution was found as separation solution.This study took pH 5.5 solution as separation solution and used real-time separation technology to separate the X and Y spermatozoa.The result showed that the motility of separated sperm reached (91±1.4)% and increased by 9.1% compared with the original,and significant differences existed between the separated sperm and the original（P<0.01）；the rate of X spermatozoa reached (73.3±1.0)%,Y spermatozoa reached (26.7±1.0)%，the difference was significant compared with the original.The real-time separation technology in the environment of acid inducement is a brief and effective technology in the separation of X and Y spermatozoa. |
| Key words: acid inducement X,Y-spermatozoa real-time separation BWW solution F body |
