| 摘要: |
| 以T型细胞质雄性不育（CMS）、化学杂交剂（CHA）诱导的雄性不育及成熟可育的花粉粒为材料，用考马斯亮蓝R250显示细胞内微丝的方法，观察分析了3种类型花粉粒内微丝结构的变化。结果表明，成熟可育花粉粒内微丝多以多聚体的形式存在，呈连续排列的网状结构且充满整个花粉粒；而其他两种不育花粉粒内，连续排列的网状结构被破坏而呈颗粒状或短棒状结构，收缩集中分布于花粉粒中部，花粉粒周缘缺乏。且T型细胞质雄性不育系的内微丝含量较化学杂交剂诱导的雄性不育花粉粒的少。 |
| 关键词: 细胞骨架 小麦 CMS CHA |
| DOI： |
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| 基金项目:国家自然科学基金项目（39770366）；国家杨凌农业生物技术育种中心基金项目 |
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| Preliminary studies on relationship between the cytoskeleton and the male sterile lines of wheat |
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| Abstract: |
| The structural characteristics of the microfilaments were revealed in sterile pollenes of CMS, CHA and mature fertile on wheat by Coomassie brilliant blue R250. The results indicated that mature fertile pollens were full of the reticular structure compositng by polymerized microfilaments, and that the reticular structure of microfilaments were disrupted and broke in two sterile pollens of CMS and CHA,then centrataed in the middle part of the pollens,no distribution near outmargin of pollens. The microfilaments in pollen of CMS(T-type) were lower than those of CHA. The study provided new method and thoughts for research mechanism of pollen abortion on CMS and the function of cytoskeleton and the relationship between them in wheat pollens. |
| Key words: cytoskeleton wheat CMS CHA |
