| 摘要: |
| 以低次烟叶为材料，研究了烟叶蛋白质最佳提取工艺。采用L9（34）正交试验设计研究了烟叶磨浆工艺。结果表明，磨浆工艺的最佳工艺参数为固液比（烟叶∶水）1∶17，提取温度60 ℃，提取液pH 8.0，磨浆2次。采用响应曲面分析法研究了烟叶蛋白碱溶工艺，得到其最佳工艺参数为温度60 ℃，pH 8.0，时间60 min，搅拌条件下碱溶3次。烟叶蛋白酸沉操作中，在pH 3.0，4 ℃下静置8 h效果最佳，最终烟叶蛋白提取率为76.62%。 |
| 关键词: 低次烟叶 蛋白质 响应面 提取率 |
| DOI： |
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| 基金项目:广东省烟草专卖局重大科研项目(粤烟科[2003]17号) |
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| Study on protein extraction technology from discarded tobacco leaf |
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| Abstract: |
| The optimum extracting conditions of protein in discarded tobacco leaf was studied.The results showed that the optimum technology conditions for tobacco leaf grinding were as follows:the ratio of 1(tobacco leaf)∶17(water),temperature 60 ℃,pH 8.0 and grinding twice with orthogonal experiment design methods L9（34）.The optimum extracting conditions of protein were 60 ℃，pH 8.0,60 min and extracting three times with agitation by Response Surface Methodology.And the optimum conditions of depositing protein with acid were pH 3.0,4 ℃ for 8 h.The extraction rate reached 76.62% under the optimal conditions. |
| Key words: discarded tobacco leaf protein response surface methodology extraction percent |
