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氧化三甲胺对南美白对虾生长、养分消化率和消化酶活性的影响
朱兴一1, 夏枚生1, 胡彩虹1
浙江大学 饲料科学研究所
摘要:
选用经基础日粮暂养1周后尾均初始体重为(1.01±0.05) g的健康南美白对虾为试验动物,以基础日粮为对照组,在基础日粮中分别添加2,4,6和8 g/kg氧化三甲胺(TMAO)为试验组,每组均设3个重复,饲喂8周,研究TMAO对南美白对虾生长、养分消化率和消化酶活性的影响。结果表明:(1)4,6和8 g/kgTMAO添加组南美白对虾的终末平均体重和增重率显著高于对照组(P<0.05);2和4 g/kgTMAO添加组南美白对虾的饵料系数显著低于对照组(P<0.05),而蛋白质效率显著高于对照组(P<0.05)。(2)添加4和6 g/kgTMAO均可显著提高南美白对虾对干物质、粗蛋白和灰分的表观消化率(P<0.05)。(3)2,4和6 g/kgTMAO添加组南美白对虾的肝胰脏蛋白酶、肝胰脏淀粉酶和肠道淀粉酶活性与对照组相比均显著提高(P<0.05);4和6 g/kgTMAO添加组南美白对虾的肠道蛋白酶活性与对照组相比也显著提高(P<0.05);8 g/kg添加组仅肝胰脏蛋白酶活性与对照组相比显著提高(P<0.05);添加TMAO对胃蛋白酶和肝胰脏脂肪酶活性无显著影响(P>0.05)。
关键词:  氧化三甲胺  南美白对虾  生长  养分消化率  消化酶活性
DOI:
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基金项目:
Effects of trimethylamine oxide on growth,apparent digestibility coefficient and activity of digestive enzymes in Penaeus vannamei Juveniles
Abstract:
Common Pacific white shrimp (Penaeus vannamei) Juveniles with body weight of (1.01±0.05) g were fed diets supplemented with trimethylamine oxide (TMAO) at a level of 0,2,4,6 and 8 g/kg respectively for 8 weeks after a week of temporary feeding with basal diets.The results were as follows:(1) Addition of 4,6 and 8 g/kg TMAO increased (P<0.05) the final body weight and weight gain rate.Addition of 2 and 4 g/kg TMAO decreased (P<0.05) the feed conversion rate and the protein efficiency rate of the juveniles.(2) Adding 4 and 6 g/kg TMAO significantly improved (P<0.05) the apparent digestibility coefficients of dry matter,crude protein and ash.(3) The activity of hepatopancreas protease,hepatopancreas amylase and intestine amylase of the groups adding 2,4 and 6 g/kg TMAO and the activity of hepatopancreas protease of the group adding 8 g/kg TMAO were higher (P<0.05) as compared with those of the control group.Addition of 4 and 6 g/kg TMAO also significantly improved (P<0.05) the activity of intestine protease.There was no significant effect on the activity of stomach protease and hepatopancreas lipase by adding TMAO to the diets (P>0.05).
Key words:  trimethylamine oxide  Penaeus vannamei  growth  apparent digestibility  activity of digestive enzyme