| 摘要: |
| 以甘蓝型白花油菜及其分离群体为材料，对甘蓝型油菜白花性状的遗传规律进行了研究。结果表明，白花对黄花是一对由不完全显性基因（WW）控制的不完全显性遗传性状；利用集群分离法（BSA）对该白花基因进行RAPD分析，在1 200个随机引物中，获得了引物S1092（5-CCC AGG CTA C-3）,在白花基因库和黄花基因库间扩增出多态性产物S-10921250；对该分离群体及其姊妹系和其他白花油菜进行的单株验证表明，S-10921250与甘蓝型油菜白花基因相连锁，遗传距离为0.84 cM。 |
| 关键词: 甘蓝型油菜 白花 分子标记 RAPD 集群分离法 |
| DOI： |
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| 基金项目:陕西省自然科学研究计划项目［2001SM32］ |
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| RAPD markers linked to white-petal gene in Brassica napus L. |
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| Abstract: |
| White-petal (W) rapeseed (Brassica napus L.) and its segregation population were used as experiment materials to study the inheritance of its white-petal traits.The result showed that the petal color character of W vs Y (yellow-petal) was controlled by one pair incomplete dominant gene WW.Bulked segregation analysis (BSA) was employed to identify random amplified polymorphic DNA (RAPD) markers linked to the white-petal gene.A total of 1 200 arbitrary 10-mer oligonucleotide primers were screened on the DNA of W and Y bulks.Primer S1092（5-CCC AGG CTA C-3）gave polymorphism between the two bulks.DNA from Individual plants of the two bulks,their sister lines and the other white-petal rapeseed (Brassica napus L.) were then used as template for screening with the primer S1092 and the 1.25 kb DNA polymorphism generated by the primer S1092 were verified linking with the white-petal gene.The genetic distance was 0.84 cM between RAPD marker and white-petal gene. |
| Key words: Brassica napus L. white-petal molecular marker RAPD BSA |
