| 摘要: |
| 采用改进的CTAB法，研究了与PCR相匹配的转基因小麦单株微量DNA的快速提取方法。结果表明，该提取方法简便、快速、有效，提取的DNA产量和质量完全可以用于PCR扩增。对标准PCR的反应体系进行优化，建立了最佳的转外源高赖氨酸基因小麦植株的PCR扩增体系，即10×Reaction Buffer 2.5 μL，MgCl2 25 mmol/L，dNTPs 2.5 mmol/L，模板DNA 30～60 ng，引物1.2 μmol/L，Taq酶1 U，加ddH2O至25 μL，优化的PCR反应体系显著提高了转基因小麦植株筛选和鉴定的效率。 |
| 关键词: 转基因小麦 DNA提取 PCR 反应体系优化 |
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| Rapid confirmation of the transgenic wheat lines by polymerase chain reaction (PCR) |
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| Abstract: |
| A new rapid DNA extraction method was developed for transgenic wheats identified by PCR.The result showed that this method was convenient,quick and effective.The quality of DNA extracted using this method was suitable for PCR analysis.An optimal reaction sytstem suitable for alien lysine-rich gene PCR was also established in this research:10×Reaction Buffer 2.5 μL,MgCl2 25 mmol/L,dNTPs 2.5 mmol/L,Genmonic DNA 30-60 ng,primers 1.2 μmol/L,Taq enzyme 1 U,ddH2O to 25 μL. |
| Key words: transgenic wheat DNA extration PCR optimization of the reaction system |
