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| 摘要: |
| 在对黑曲霉WM20-11产酸性β-甘露聚糖酶的培养基组分和培养条件单因素研究的基础上，设计了Plackett-Burman试验选取主要影响因素，并进行响应面分析。结果表明，黑曲霉WM20-11产酸性β-甘露聚糖酶的最适培养基和发酵条件为：水11.7 mL，麸皮9.0 g，豆饼粉1.0 g，魔芋粉0.98 g，玉米浆0.375 mL，(NH4)2SO4 12.5 g/kg，CaCl2 1.0 g/kg，MgSO4 1.0 g/kg，KH2PO4 2.5 g/kg(相对于固体料)，自然pH；28 ℃固体静置培养96 h，期间翻曲2～3次。在此条件下，菌株产酶活性可达1 337 IU/g干曲。 |
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| 基金项目:江南大学校级科研项目(210000-52212052) |
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| Study on the solid-state fermentation of acidic β-mannanase |
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| Abstract: |
| The effects of the nutrition ingredients and cultural conditions on the production of acidic β-mannanase by Aspergillus niger WM20-11 were studied in this paper.The optimal fermentation medium contained 9.0 g of wheat bran,1.0 g of soybean flour,0.98 g of konjac powder,0.375 mL of steep liquor,12.5 g/kg (NH4)2SO4,1.0 g/kg CaCl2，1.0 g/kg MgSO4，2.5 g/kg KH2PO4,and 11.7 mL of H2O.The highest β-mannanase activity was 1 337 IU/g dry matter by solid-state fermentation at 28 ℃ for 96 hours. |
| Key words: β-mannanase Aspergillus niger solid-state fermentation response surface analysis |
