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菠菜种子超干贮藏后抗老化作用的生理特性
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摘要:
从发芽率、保护酶系活性、脱氢酶活性、丙二醛含量和种子蛋白等方面对菠菜种子超下贮藏效果进行了研究。结果表明,超干孵:藏町以提高种子的抗老化劣变能力。菠菜种子含水量为3.3%~5.4%时,表现出较强的抗老化和劣变能力及较好的耐藏性。超干和老化处理后菠菜种子的SOD,POD.CAT和脱氢酶活性显著高于原始含水量种子,而丙二醛含量显著低于原始含水量种子。不同含水量菠菜种子的SDS—PAGE蛋白质谱带尤显著差异。
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基金项目:国家“十五”科技攻关重大专项(2004BA516A09);西北农林科技大学2004年科研专项(042M083)
Studies on spinach seeds' physiological characteristics of aging-resistance after ultra-dry storage
Abstract:
Spinach seeds were used to investigate the ultra-dry effect of aging-resistance.The results showed that ultra-dry seeds became more tolerant to aging conditions.When spinach seeds were dried to about 3.3%-5.4% of moisture content,they became more tolerant to aging and were easier to store.Under the same aging condition,the activity of SOD,POD,CAT and dehydrogenase of ultra-dry seeds were higher while Malonadehyde (MDA) were lower than seeds with original moisture content.The protein of SDS-PAGE bands were almost the same in spinach seeds of different moisture content.
Key words:  spinach seeds  ultra-dry storage  SOD  POD  CAT  dehydrogenase  MDA  SDS-PAGE