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| 摘要: |
| 设计了1对特异性引物,应用降落PCR法对减蛋综合症病毒(EDSV)六邻体蛋白基因进行了扩增。将扩增产物克隆至pMD18-T载体中,经酶切和PCR鉴定后,初步证明了目的片段的正确性。进一步经核苷酸序列测定表明,所扩增的基因片段长度为2.74 kb,共编码910个氨基酸,与由EDSV弱毒株(AA-2)全基因组的的HindⅢ酶切片段测序所得到的六邻体结构基因推测的氨基酸序列相比,有11处氨基酸发生了变异。 |
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| 基金项目:河南省自然科学基金资助项目(0214040027) |
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| Cloning and sequence analysis of hexon-encoding gene of egg drop syndrome virus |
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| Abstract: |
| Hexon-ecoding protein gene of egg drop syndrome virus (EDSV) was amplified by touchdown PCR(TD-PCR) and was cloned into pMD18-T vector.The recombinant plasmids were identified by digested restriction endonuclease and PCR.The sequence analysis showed that the nucleotide sequence of hexon protein gene was 2.74 kb and encoding a protein of 910 amino acids,11 amino acids were different from the hexon protein sequenced from the HindⅢ-D fragment of egg drop syndrome virus AA-2 strain. |
| Key words: EDSV hexon gene cloning sequence analysis |
