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| 摘要: |
| 采用酶消化法和组织块法对小鼠、奶山羊、奶牛和家猪的表皮干细胞进行了分离、培养。结果表明：酶消化法适于小鼠表皮干细胞的分离，所获得的小鼠表皮于细胞在维持了两代之后自发分化为神经样细胞；奶山羊和奶牛不论用酶消化法还是组织块法都町以获得大量活力相对较好、表皮干细胞特性维持较久的细胞，奶山羊表皮细胞采用Ⅳ型胶原分选，用有血清培养液培养，经免疫细胞化学鉴定为表皮干细胞，可持续传至少9代，随着代数增高，细胞活力减弱。 |
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| 基金项目:国家“863”项日（2002AA216161）；国家自然科学基金项目（30200137）；教育部重大专项（03160） |
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| Isolation,purification,culture and identification of epidermal stem cells |
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| Abstract: |
| The isolation and culture of putative epidermal stem cells by way of enzyme-digesting and direct-tissue-culturing from mouse,goat,milk cow and pig were conducted in this study.Results indicated that enzyme-digesting was more suitable for the isolation of mouse epidermal stem cells which self-differentiated into neural cells after two passages of in vitro culturing while cells from goat and cow lived more vigorously and maintained longer characteristics of epidermal stem cells than those from mouse and pig either by enzyme-digesting or tissue-culturing.Epidermal stem cells from goat were selected by collagen Ⅳ,cultured in serum-containing medium,identified by immunocytochemical staining and could be subcultured to at least passage nine but the cells grew weaker as the passage continued. |
| Key words: epidermal stem cell isolation culture identification |
