| 摘要: |
| 从浓缩苹果汁生产原料中分离出多种棒曲霉素产生菌,形态学初步鉴定表明其均为青霉属和曲霉属真菌。为确定其分类地位,又用ITS-DNA测序鉴定进行了验证。将ITS-rDNA提取后进行PCR扩增,测序后提交到EuropeanMolecularBiologyLaboratory(EMBL)核酸数据库,通过Blast程序,将ITS-rDNA序列与Genebank中的核酸序列进行对照,结果确定其确为青霉属与曲霉属菌株。 |
| 关键词: 棒曲霉素 扩张青霉 ITS-rDNA PCR 浓缩苹果汁 |
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| 基金项目:国家“十五”科技攻关项目(2001BA51A15)；科技部西部专项(2001BA901A19) |
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| Identification of dominating patulin-producing strain by ITS-DNA sequence analysis in apple juice concentrates |
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| Abstract: |
| Several patulin-producing strains isolated from raw material of apple juice concentrates were identificated in morphology,in which all of them were penicillium and aspergillus.Then ITS-rDNA sequence analysis was used to confirm it.The ITS-rDNA sequence was submitted to european molecular biology laboratory (EMBL)，and contrasted with DNA sequence in genebank and confirmed that they were penicillium and aspergillus. |
| Key words: patulin Penicillium expansum ITS-rDNA PCR apple juice concentrate |
