| 摘要: |
| 以小麦愈伤组织为受体材料，用基因枪法将报告基因GUS和目的基因Psag12-IPT导入小麦中。GUS基因表达检测表明,金粉用量越高，转化频率越高；质粒DNA用量以2.0 μg/枪最佳；随着愈伤组织培养时间的延长，GUS基因瞬时表达呈明显下降趋势，至第28天以后趋于稳定。另外，GUS基因瞬时表达检测应在24 h内进行。 |
| 关键词: 转基因小麦 基因枪 愈伤组织 GUS基因 瞬时表达 |
| DOI： |
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| 基金项目:国家转基因专项(JY03-B-23-02)；陕西省科技攻关项目(2003K03-G1-04) |
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| Factors of gene transformation affecting wheat callus |
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| Abstract: |
| The plamid pcMA35-1 containing both GUS gene and Psag12-IPT gene was driven by CAMV35S promoter into wheat callus using ballistic method.Parameters affecting transformation efficiency were studied by GUS transient expression.The result showed that it had a remarkable role in the transformation efficiency at a relatively high gold particle amount per bombardment and it was best when coated by 2.0 μg DNA.The expression efficiency of GUS gene reduced with the increase of callus culture time and remained stable after 28 d.The examination of GUS gene transient expression should be at 1 d and the examination of GUS gene stable expression should be after 28 d. |
| Key words: transgenetic wheat ballistic wheat callus (Triticum aestivum L.) GUS gene transient expression |
