| 摘要: |
| 用免疫细胞化学方法,对隆肛蛙与北方山溪鲵不同发育时期卵泡c-myc和c-fos的表达产物进行了定位检测。结果表明,Myc在2种动物Ⅰ-Ⅱ期卵母细胞和滤泡细胞中表达较强，以后逐渐减弱;Fos在2种动物卵泡发育初期表达很弱，在Ⅲ-Ⅳ期卵泡中表达增强。上述结果说明,c-myc在2种动物卵母细胞发育初期起重要作用，而c-fos在发育中后期起重要的调控作用，2种原癌基因可能通过调节类固醇激素的合成与分泌来调控卵母细胞的发育。 |
| 关键词: 隆肛蛙 北方山溪鲵 c-myc c-fos 卵巢 |
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| 基金项目:陕西省自然科学基金项目(2001SM26)；延安大学专项基金项目(YD2003-190) |
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| Expression of c-myc and c-fos in ovary of the Rana quadranus and Batrachuperus tibetanus |
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| Abstract: |
| Immunocytochemical localization was investigated at different developmental stages of the follicles of the Rana guadranus and Batrachuperus tibetanus using antibodies against Myc and Fos.The results indicate that Myc is expressed strongly in the oocyte and follicle cells at the stagesⅠ-Ⅱ,then more weakly at the stages Ⅲ-Ⅳ.Fos is weakly expressed in the oocyte at early stages,but more strongly at stages Ⅲ-Ⅳ.These results prove that for Rana quadranus and Batrachuperus tibetanus,c-myc plays a key role in the early development of oocyte,and c-fos in the medium and upper stages of oocyte.The two proto-oncogenes may regulate the oocyte development of Rana quadranus and Batrachuperus tibetanus by regulating the synthesis and secretion of sex steroids. |
| Key words: Rana guadranus Batrachuperus tibetanus c-myc c-fos ovary |
