| 摘要: |
| 以84K杨为研究对象，通过建立叶片再生系统及卡那霉素敏感性试验,确立了稳定高效的基因转化受体系统。结果表明,以MS培养基为基本培养基,0.5～1.0 mg/L 6-BA+0.01～0.1 mg/L NAA组合时，叶片出芽率不到50%，每叶平均生芽数5～6个；1.0～1.5 mg/L 6-BA+0.02 mg/L 2,4-D组合时，叶片出芽率达90%～100%，每叶平均生芽数约20个。用后者附加不同质量浓度梯度卡那霉素，确定叶片外植体芽诱导卡那霉素临界筛选质量浓度为20 mg/L。利用对84K杨芽生根效果较好的培养基GMS+0.01 mg/L NAA+0.25 mg/L IBA，附加不同质量浓度梯度卡那霉素，筛选出芽生根卡那霉素临界质量浓度亦为20 mg/L。 |
| 关键词: 84K杨 基因转化 受体系统 不定芽诱导 外植体 |
| DOI： |
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| 基金项目:陕西省自然科学基金项目(2004C101) |
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| Establishment of gene transformation receptor system for poplar 84K |
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| Abstract: |
| Through the establishment of high frequency regeneration system for leaf and kanamycin sensitivity tests,a stable and efficient gene transformation receptor system for poplar 84K was set up,which laid a sound foundation for Agrobacterium-mediated transformation of disease resistance gene by means of leaf disc method.The results indicated that budding percentage was less than 50% and average adventitious buds per leaf was 5-6 when basal MS medium supplemented with 0.5-1.0 mg/L 6-BA+0.01-0.1 mg/L NAA.While 1.0-1.5 mg/L 6-BA+0.02 mg/L 2,4-D were added to MS medium,90%-100% budding percentage and 20 average adventitious buds per leaf were obtained.Utilizing the latter medium,adding different concentrations of kanamycin,the optimal selecting pressure 20 mg/L of kanamycin for adventitious bud was confirmed.GMS+0.01 mg/L NAA+0.25 mg/L IBA was better to induce root,by which the optimal selecting pressure for root induction was also 20 mg/L of kanamycin for poplar 84K when adding kanamycin different concentrations. |
| Key words: poplar 84K gene transformation receptor system adventitious bud induction explant |
