摘要: |
在对芥蓝C100-12×甘蓝秋50-Y7杂交组合构建F2作图群体进行RAPD检测的基础上,分别对4个O12,R13,Q14,P17进行了延长1个碱基的研究,其中O12和P17能将部分特异RAPD标记转化为稳定性更强的ERPAD标记,但对R13和Q14随机引物未找到相应的ERPAD标记。 |
关键词: RAPD标记 ERPAD标记 甘蓝 |
DOI: |
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基金项目:农业部蔬菜遗传与生理重点实验室项目 |
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Studies of transforming 2 RAPD markers based on F2 population from B.oleracea var.capitata×B.oleracea var. alboglabra into ERPAD markers |
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Abstract: |
Four random primers O12,R13,Q14,P17 were researched by screening 3′-end-extended random primers,in order to enhance the stability and specificity of RAPD markers of F2 population from B.oleracea var.capitata×B.oleracea var.alboglabra.The stability of ERPAD marker was higher than that of the original RAPD markers of O12 and P17,However,such ERPAD marker was not derived from R13 and Q14. |
Key words: RAPD extended random primer amplified DNA(ERPAD) cabbage(Brassica oleracea var.capitata) |