| 摘要: |
| 对含猪瘟病毒E2蛋白A/D抗原区基因插入的重组酵母菌株进行诱导表达，通过对诱导时间、重组酵母菌株、菌体密度、培养液pH、甲醇剂量等培养条件与表达产量关系的分析，对重组蛋白的表达条件进行了优化。优化后的条件为：28～30 ℃,225 r/min振荡培养至BMGY中菌体OD600值为3.0～3.5时,将菌体转入相当于4倍体积BMGY,pH为6.0的BMMY培养基中培养72 h，每24 h加入甲醇至终浓度为总体积的0.5%～1.0%。在此优化条件下，重组蛋白的表达量可达275.38 μg/mL，比较温度对重组蛋白降解率的影响后发现，培养物上清液应保存于-20 ℃。 |
| 关键词: 巴斯德毕赤酵母 猪瘟病毒E2蛋白 A/D抗原区 表达条件 条件优化 |
| DOI： |
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| 基金项目:国家863高技术发展计划项目(2001AA249012) |
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| Secretion expression conditions optimization of E2 protein A/D antigenic domain of hog cholera virus in Pichia pastoris |
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| Abstract: |
| An option evaluation model on conglomerate M&A is given for those companies that have high uncertainty of future development perspective, by using a precise mathematical model.The result indicates that under several suitable parameters, the opportunity value of waiting to further invest after merging such a company is very large and makes it an important fact in M&A decision-making. |
| Key words: Pichia pastoris A/D antigenic domain expression conditions optimation |
