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转基因棉对高抗Bt棉棉铃虫生长发育及活动频率的影响
高聪芬1, 沈晋良1, 钱 燕1
南京农业大学 农业部病虫监测与治理重点开放实验室
摘要:
以敏感品系棉铃虫在苏棉12和玉米上的生长发育及活动频率为对照,研究了新棉33B对高抗Bt棉棉铃虫生长发育及活动频率的影响。结果表明,高抗品系棉铃虫在转基因棉中后期主茎顶部嫩叶、侧枝嫩芽及嫩叶、蕾、铃上完成幼虫和蛹期的发育时间,分别比敏感品系棉铃虫在常规棉上的发育时间长13.4,10.7及7.0 d,比敏感品系在玉米上完成发育时间长13.2~20.6 d;且取食转Bt基因棉的高抗棉铃虫幼虫死亡率和活动频率高,蛹重减轻,化蛹率和羽化率降低。
关键词:  高抗Bt棉棉铃虫  转基因棉  生长发育  活动频率
DOI:
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基金项目:国家自然科学基金项目(30270889);教育部博士点基金项目(2000030703)
Effect of transgenic cotton on the development and activity frequency of high resistant strain of Helicoverpa armigera (Hübner) to Bt cotton
Abstract:
In Contrast with the development of susceptible strain of Helicoverpa armigera (Hübner) fed on SM12 and corn,the development and movable frequency of resistant strain fed on 33B were studied.The results showed the larval and pupal stages of resistant strain fed on the terminal leaf,terminal bud of lateral branch and tender leaf/bud/boll of Bt cotton (33B) were longer than those of susceptible strain fed on SM12,the development of resistant strain fed on 33B was delayed for 13.4 d,10.7 d and 7.0 d than the susceptible strain fed on non transgenic cotton,respectively;and 13.2-20.6 d than the susceptible strain fed on corn organ,respectively.Transgenic cotton was also found to cause a higher mortality and activity frequency of the bollworm larvae,lighter weight of pupae and lower pupation rate and emergence rate.The efficiency of high-dose/refugy strategy was also discussed in this paper.
Key words:  high level resistant strain of H.armigera (Hübner)  transgenic cotton  development  activity frequency