| 摘要: |
| 应用RT-PCR方法从鸡新城疫Ⅰ系疫苗接种鸡胚的脾淋巴细胞中扩增出IL-2基因cDNA。结果表明，以鸡新城疫Ⅰ系疫苗稀释50倍或100倍接种10日龄鸡胚,培养48 h的脾脏提取mRNA的扩增效果最好。测序结果显示，所扩增片段的核苷酸序列及其所编码的氨基酸序列，与已发表的IL-2基因的同源性分别为97.7%～99.8%和95.1%～99.3%。其中第8位、68位和130位氨基酸（分别是L，V和S）的变异是其他品种鸡所没有的。 |
| 关键词: 鸡IL-2基因 转录 克隆 序列分析 |
| DOI： |
| 分类号: |
| 基金项目:河南省科技攻关项目(0424050010) |
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| Transcription and cDNA cloning of chicken interleukin-2 induced chicken embryo splenocytes by Newcastle Disease viruse |
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| Abstract: |
| A cDNA encoding chicken interleukin-2 (chIL-2) was cloned from Luoyang local chicken embryo splenocytes by reverse transcription-polymerase chain reaction (RT-PCR).The results showed that the best factor for mRNA isolated from spleen cells of chicken embryo was 48 h after being inoculated with 50× or 100× diluted Newcastle Disease virues.Sequence analysis showed that the chIL-2 cDNA was 432 bp and encoded a protein 143 amino acids compared with reported chIL-2.The homology of nucleotide acid and amino acid were 97.7%-99.8% and 95.1%-99.3%,respectively.Especially the amino acids in sites 8,68 and 130 (L,V and S) were different from other strains.These provide a basis for the study of the structure and function of chIL-2 gene. |
| Key words: chicken IL-2 gene transcription cloning sequence analysis |
