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食用仙人掌芽增殖体系的建立
张正波1, 高书宝1, 李春莲1
西北农林科技大学 农学院 细胞工程实验室
摘要:
研究了基本培养基种类及外源激素对食用仙人掌芽增殖的影响。结果表明,MB基本培养基较MS基本培养基有较高的芽增殖效果。6-BA对食用仙人掌芽增殖有明显的促进作用;KT对芽增殖无明显促进作用;6-BA与KT,6-BA与NAA配合能稳定促进食用仙人掌的芽增殖和进一步发育,获得增殖率高;食用仙人掌芽增殖的最佳培养基为MB+6-BA 1.0 mg/L+NAA 0.5 mg/L。
关键词:  食用仙人掌  芽增殖  组织培养  增殖率
DOI:
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基金项目:国家杨凌农业综合试验技术研究中心资助项目
Establishment of high shoot proliferation system of edible cactus
Abstract:
The effect of media and plant hormones on shoot proliferation of edible cactus was studied using shoot proliferation induction media containing different organic substances and plant hormones in this experiment.The result shows that the rate of shoot proliferation of edible cactus on MB basic media was significantly higher than that on MS basic media,6-BA had significant positive effect on shoot proliferation of edible cactus while the effect of KT on shoot proliferation of edible cactus was very poor;that combination of 6-BA,KT and NAA could prompt proliferation and further development of shoot of edible cactus and the rate of which was very high;the best media for edible cactus shoot prolieration is MB with 6-BA 1 mg/L,NAA 0.5 mg/L.
Key words:  edible cactus  shoot proliferation  tissue culture  rate of proliferation