| 摘要: |
| 以毛白杨无菌苗的嫩茎为外植体,对5个毛白杨无性系的植株再生能力进行了比较。结果表明,5个无性系通过器官培养途径均能再生植株,但不同无性系在植株再生能力上有很大差异,茎芽数最多的4号无性系是总平均的122.7%，而最少的1号无性系仅为总平均的78.4%；2号无性系生根率可达60%，3号仅28.7%，说明茎芽分化及生根能力受遗传的控制。0.5 mg/L 6-BA适合大多数无性系的茎芽增殖和生长,平均茎芽增殖达到8.8个，但生根率仅为49.5%。1/2MS基本培养基适合各无性系的生根培养,在培养基中添加低质量浓度的生长素可以增强生根能力。毛白杨在植株再生能力方面存在明显的培养基×无性系交互作用。 |
| 关键词: 毛白杨 无性系 植株再生 组织培养 |
| DOI： |
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| 基金项目:陕西省科技攻关项目(2001K01-G7-01) |
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| Plantlet regeneration capacity of selected Populus tomentosa clones in vitro |
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| Abstract: |
| Five excellent clones of Populus tomentosa were used in this study.Sterile shoots were used as explants to induce bud differentiation and growth，as well as rooting in different medium.The potential of plantlet regeneration was compared with different clones.The results showed that all five clones of P.tomentosa could obtain regeneration plantlets by organogenesis in vitro.But a marked difference was observed in axillary shoots number and rooting rate,in which No.4 clone axillary shoot number is 122.7% of the average and No.1 only averages 78.4%.No.2 rooting rate reachs 60% and No.3 only 28.7%.It indicated that capacity of shoot proliferation and rooting was genetically controlled.The 0.5 mg/L 6-BA concentration may be the optimal value for induction and proliferation of shoot for most clones in vitro.Although average shoot number reached 8.8，rooting percentage was only 49.5%.1/2 MS basal medium was suitable to rooting for clones.The rooting capacity could be improved by supplementing low concentration auxin.At the same time there existed a visible media×clone interaction in plantlet regeneration capacity for P.tomentosa. |
| Key words: Populus tomentosa clones plantlet regeneration tissue culture |
