| 摘要: |
| 采用1 g/L胶原酶灌注猪脐静脉，消化分离内皮细胞并在适宜条件下培养。对培养细胞的生长特性进行检测，并对细胞进行第Ⅶ因子相关抗原(F-ⅦRAg)和扫描电镜形态学鉴定。结果表明，70％细胞在24 h内贴壁，第3～5天生长迅速，第4～5天长成单层。培养的细胞呈单层贴壁生长，典型铺路石状，第Ⅶ因子相关抗原(F-ⅦRAg)检测阳性，证明培养的细胞为血管内皮细胞。 |
| 关键词: 细胞培养 猪脐静脉血管内皮细胞 胶原酶 第Ⅷ因子相关抗原(F-ⅧRAg) |
| DOI： |
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| 基金项目:国家自然科学基金项目(30270988) |
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| Segregation and culture of endothelial cells from swine umbilicus veins |
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| Abstract: |
| Swine umbilical cord was sterilized by 75% ethyl alcohol about 1 min and umbilicus veins were washed by PBS.1 g/L callagenaseⅠwas poured into umbilicus vein and digested 5 min at 37 ℃.Endothelial cells obtained was collected by centrifugation,cultured in M199+20% fetal calf serum.The plating efficiency to plastic was 70%-75% for 24 hours in callagenase dispersed cells.A confluent monolayer was former by 4-5 days.The the endothelial cells looked like cobblestones and were stained positively for factor Ⅷ relative antigen,which is feature of endothelial cells.It shows cells cultured are umbilicus veins cells. |
| Key words: cell culture swine umbilicus veins endothelial cells callagenase factor Ⅷ relative antigen |
