| 摘要: |
| 从4～15周龄的牛胎儿生殖嵴中分离得到牛原始生殖细胞，以STO(一种建系的小鼠胎儿成纤维细胞)及MEF(小鼠胎儿成纤维细胞)为饲养层抑制分化培养，其中1个细胞系传至4代。研究发现，STO较MEF更有利于牛类EG细胞的分离与培养，体长小于5 cm的胎儿适合牛EG细胞分离与克隆。同时观察到这些细胞在体外进行自发性分化，可形成上皮样细胞、神经样细胞和成纤维样细胞。 |
| 关键词: 原始生殖细胞 胚胎干细胞 胚胎生殖细胞 牛 |
| DOI： |
| 分类号:S827 |
| 基金项目:国家自然科学基金资助项目(30070374) |
|
| Isolation and maintenance of bovine primordial germ cells |
|
LI Rui-biao~1,CHEN Hong~,LI Rui-biao~1,CHEN Hong~,LI Rui-biao~1,CHEN Hong~
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| Abstract: |
| The objective of this study was to isolate and maintain bovine PGCs from the genital ridge of 4-15 weeks gestation aborted fetuses.PGCs were plated on mouse STO or MEF feeder so as to maintain and clone.One bovine EG-like cells line was passaged to 4 passages.Bovine EG-like cells lived better on STO feeder than MEF feeder in our experiments,and bovine fetues,less than 5 cm crown-rump length,were beneficial to isolate and clone EG-like cells.We also observed these EG-like cells spontaneously differentiated into epithelial-like,neuron-like and fibroblast-like cells. |
| Key words: primordial germ cells embryonic stem cells embryonic germ cells bovine |
