| 摘要: |
| 采用RT-PCR方法成功地从人胎盘组织扩增出hIGF-Ⅱ基因。将其连入表达载体pET30a(+)质粒中，SDS-PAGE结果证明，pET30a(+)-hIGF-Ⅱ在大肠杆菌BL21(DE3)中获得高效表达，目的蛋白占总菌体蛋白35％左右。 |
| 关键词: 胰岛素样生长因子Ⅱ 基因克隆 基因表达 |
| DOI： |
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| 基金项目:国家“863”计划项目(2001AA213081)，西北农林科技大学重点专题基金项目 |
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| Cloning and expression of human insulin-like growth factorⅡ gene |
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| Abstract: |
| Human IGF-Ⅱ gene was successfully amplified from human placenta with RT-PCR method,then cloned it into expression vector：pET30a(+).pET30a(+)hIGF-Ⅱ was highly expressed in BL21(DE3) and the recombinant hIGF-Ⅱ was about 35% of total proteins measured with SDS-PAGE. |
| Key words: hIGF-Ⅱ gene cloning gene expression |
