| 摘要: |
| 利用60Co-γ射线与HNO2复合处理辣椒种子,结果表明,M1代的损伤效应随着复合处理剂量的增加而增大;发芽势、根长表现累加效应,成株率表现协和效应,但对最终发芽率影响很小;复合处理最优诱变剂量为30(1×10)Gyγ射线与0.05 nrmol/L的HNO2浸种70 min;复合处理最优诱变剂量的早期形态预测指标是M1根长为对照的54%;诱变处理对发芽势、成株率、株高和根长的效应趋势一致,其相关系数均达到显著水平.据此认为,发芽势、成株率和株高可作为早期测定复合处理生物学效应的可靠参考指标. |
| 关键词: 辣椒 γ射线 HNO2 复合处理 诱变效应 |
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| 基金项目:陕西省自然科学基金项目(2001SM11);陕西省农业生物技术重点实验基金项目 |
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| Mutagenic effects of the combined treatments with 60Co-γ ray and HNO2 on the peppers in M1 |
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| Abstract: |
| Experiment was conducted to study mutagenic effects of the single and combined treatment with 60Co-γ ray and HNO2 on dry seeds of peppers in M1.The results showed that the combined treatment inhibited the germination rate at 7th day in different degree but did not affect germination rate eventually and that additive as well as synergistic effects were observed in the M1 generation treated with 60Co-γ rays and HNO2.The additive effect was observed on the root length and the germination rate,the synergistic effect was observed on the rate of survival plant.54% of root length in M1 could be used for predicting effect in M2 under the best combined treatment.The treatment of 30（1×10）Gy γ ray+70 min (0.05 mol/L HNO2) was the best one.There were the significant relationship between the rate of survival plant,the plant height,the germination rate and the root length. |
| Key words: pepper 60Co-γ ray HNO2 combined treatment effect of induced mutation |
