| 摘要: |
| 以芥菜（Brassica juncea Coss）雪里蕻和圆叶芥为试材，在携带潮霉素磷酸转移酶基因和CaMV Bari-1基因Ⅵ的根癌农杆菌菌株GV3101的介导下，采用植株原位真空渗入遗传转化技术，将外源基因潮霉素磷酸转移酶基因和CaMV Bari-1基因Ⅵ导入芥菜。采收T1种子，经潮霉素抗性筛选和PCR分子检测，证实获得了转基因植株。 |
| 关键词: CaMV基因Ⅵ 真空渗入法 遗传转化 根癌农杆菌 芥菜 |
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| 基金项目:国家自然科学基金项目(39770522)，教育部优秀年轻教师资助项目，科技部杨凌生物育种中心基金资助项目的部分内容(1999-18B) |
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| Transformation of mustards (Brassica juncea Coss.) by the plant in situ vacuum infitration |
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| Abstract: |
| Mustards （Brassica juncea Coss.） ecotype Xuelihong and Yuanyejie were transformed with in planta vacuum infiltration by an Agrobacterium tumefaciens strain GV3101 carrying hygromycin resistance gene and CaMV Bari-1 gene Ⅵ.The T1 seeds of mustards were screened through medium with 25 mg/L hygromycin and some resistant seedlings were obtained.PCR molecular analyses showed that target genes were transferred to the plant cells. |
| Key words: CaMV gene Ⅵ In planta vacuum infiltration genetic transformation Agrobacterium tumefaciens Brassica juncea |
