| 摘要: |
| 利用仙客来(Cyclamen persicum)种苗进行组培快繁建立良种无性系，对初培养、继代、生长及生根各时期的适宜配方进行了研究，并对继代期间不定芽分化等进行了数量性分析。结果表明：在初培养时，6-BA和2，4-D的配合对不定芽的分化启动是必须的。在继代过程中，激素、不同品种和单株对芽分化能力和苗再生都有显著影响。在组培苗生根过程中品种间的差异极显著。不定芽生根前经一代生长壮苗后其生根状态及生长状态均优于未经生长直接生根的植株。利用单株种苗建立无性系可成为仙客来无性快繁的一条新路。 |
| 关键词: 仙客来 组织培养 单株无性系 数量性分析 |
| DOI： |
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| 基金项目:山东省科技攻关项目(012010121) |
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| Fast propagation of cyclamen clones from single plant through tissue culture |
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| Abstract: |
| Clones of cyclamen were fast propagated through tissue culture.Studies were made on formula of mediums suitable for different culture stages such as primary culture,successive culture,growth and rooting.The results showed that,in order to start differentiation of adventitious buds,it was necessary to add both 6-BA and 2,4-D during primary culture.During successive culture,hormones,sugar sources,cultivar and individual plant all had significant influence on differentiation of buds and regeneration of shoots.During the rooting stage,there was highly significant difference between cultivars and cultivars showed different sensitivity to the concentration of hormone and its range.Before rooting,adventitious buds went through another run of culture to make them stronger.They showed better performance in rooting and growth.Obtaining clones from single plant and then making selective fast propagation is a new way to propagate cyclamen. |
| Key words: cyclamen tissue culture seedling tissue single plant C quantitative analysis |
