| 摘要: |
| 胚胎干细胞是由早期内细胞团分离的一种多潜能细胞，在体外分化抑制培养时，可保持未分化状态而无限增殖。一旦撤除分化抑制因素，或添加适当的诱导剂，ES细胞可分化为内胚层、中胚层和外胚层的多种类型的特化细胞。文章综述了体外定向诱导ES细胞分化为造血细胞、心肌细胞、神经细胞、脂肪细胞、胰岛细胞、内皮细胞、上皮细胞、成骨细胞、软骨细胞和黑素细胞等的途径和方法。 |
| 关键词: 胚胎干细胞 定向诱导分化 生物工程 |
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| 基金项目:国家“973”项目(G1999054301-5)；国家自然科学基金资助项目(39970363) |
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| The study progress of induced commitment and differentiation of ES cells in vitro |
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| Abstract: |
| Embryonic stem cells are pluripotent cells derived from the inner cell mass of early blastrocyts that can be propagated indefinitely in the primitive undifferentiated state under in vitro differentiation-inhibited culture.Under appropriate conditions embryonic stem cells have been shown to differentiate in vitro to multiple cell types of endoderm,mesoderm and ectoderm.This paper reviews the pathways of ES cells in vitro being committed and differentiated to hematopoietic cells,cardiomyocytes,neural cells,adipocytes,pancreatic islets,endothelial cells,epithelia,osteoblasts,chondroblast and melanocytes. |
| Key words: embryonic stem cells induced commitment and differentiation biotechnology |
