| 摘要: |
| 针对人骨形成蛋白3基因的cDNA序列设计引物，通过反转录PCR技术，从人骨纤维肉瘤组织中扩增出目的片段。从胶中回收目的片段，将其与pMDl8-T载体连接，转化大肠杆菌DH5α后，经HindⅢ酶切鉴定，进行测序。结果显示所得到的片段大小为845 bp，与所设计的序列同源性为100%。说明已经成功地扩增、克隆了人骨形成蛋白3(hBMP-3)基因cDNA的大部分序列。 |
| 关键词: 骨形成蛋白 RT-PCR 人骨形成蛋白3基因 |
| DOI： |
| 分类号: |
| 基金项目:国家863高技术计划项目(2001AA213081) |
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| Cloning of partial cDNA of human bone morphogenetic protein 3 (hBMP-3) gene by RT-PCR |
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| Abstract: |
| A pair of primers for the amplification of partial cDNA sequence of human bone morphogenetic protein 3 gene were designed,the target fragment was amplified by RT-PCR from the tissue of human bone fiber sarcoma,then was purified from agarose gels and integrated into pMD18-T vector.After being transformed into E.coli.DH5α,tested by HindⅢ restriction endonuclease digestion,the positive clone was sequenced.The sequencing result indicated that the size of the fragment cloned is 845 bp,and its sequence homology with the designed one is 100%.The conclusion can be made that the major part of the cDNA of human bone morphogenetic protein 3 (hBMP-3) gene has been successfully amplified and cloned. |
| Key words: BMP RT-PCR hBMP-3 gene |
