| 摘要: |
| 以宁夏农科院选育的新品种宁杞1号3年生树为试材，对影响枸杞胚细胞悬浮培养及其再生系统建立的培养基与激素配比进行了研究。结果表明，以人工授粉后18 d的幼果，剖取成熟胚作为外植体，选择MS＋6BA 1.0 mg/L IBA 0.5 mg/L培养基诱导愈伤组织，经单细胞悬浮培养建立了稳定的细胞系；MS＋6BA 0.2 mg/L培养基较为适宜枸杞胚状体萌发成苗，该再生苗转移到MS＋NAA 0.2 mg/L生根培养基上，诱导生根并获得完整植株；共移栽成活14株，成活率达38.8%。 |
| 关键词: 宁夏枸杞 胚培养 细胞悬浮培养 植株再生 |
| DOI： |
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| 基金项目:宁夏回族自治区科委重点资助项目(970117014) |
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| Establishment of the suspension culture on cell line from embryo in Lycium barbarum L. |
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| Abstract: |
| The three-year-old tress of Lycium barbarum L.cv.Ningqi No.1 were used as plant materials.The MS mediums with different hormone composition were studied to compare their effect on establishment of the supension culture on cell line from embryo in Lycium barbarum L.The results showed that the 18-day-old mature embryos from the fruit pollinated were taken out as explants,and inoculated in the best MS+6BA 1.0 mg/L+IBA 0.5 mg/L medium for inducing callus,the stable cell lines by the cell suspension culture were set up,MS+6BA 0.2 mg/L as rooting medium was used to culture the formed seedings and 14 plantlets transferred to the field were obtained,the regeneration efficiency was 38.8%. |
| Key words: Lycium barbarum L. embryo culture cell suspension culture plant regeneration |
