| 摘要: |
| 用肿囊腐霉的孢子悬液分别对脱毒马铃薯抗病自交系F1和感病自交系F的扦插苗进行接种，并测定了接种后根中苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）、超氧化物歧化酶（SOD）的活性和可溶性蛋白的含量。结果表明，接种后，脱毒马铃薯抗病自交系F1的PAL活性曲线呈现2个峰，而感病自交系F的PAL活性曲线呈现1个峰；病原菌感染早期，F1，F的POD和SOD活性都有不同程度的下降，但到后期，F1的POD和SOD活性均迅速回升，而F的则继续维持低水平；F1的可溶性蛋白含量在接种后呈现1个峰，到后期维持较高含量，而F的可溶性蛋白含量持续减少。 |
| 关键词: 马铃薯脱毒苗 青枯病 根部生理变化 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目资助 (70041043) |
|
| Study on the physiological changes of dehydrated virus potato root during infection with Pythium inflatum |
|
|
| Abstract: |
| Dynamic changes in activities of phenylalanine ammonialyase,peroxidase,superoxide dismutases and soluble protein contents were analysed during infection by pythium inflatum in resistant inbred line F1 and susceptible inbred line F of dehydrated virus potato.After inoculation,the activities of the PAL of F1 showed two peaks,while that of F showed only one peak.In initial stage of penetration by the fungus,POD and SOD activities in both resistant and susceptible inbred lines showed a trend of decrease,while in late stage of the infection F11 displayed greater increase of POD and SOD.But the activity of both enzymes in F still stayed in a lower level.In initial stage of infection,soluble protein contents of F1 showed one peak and become significantly higher on the contrary.But that of F showed a trend of decrease. |
| Key words: dehydrated virus potato nbasal stalk rot phenylalanine ammonialyase perxidase superoxide dismutases soluble protein contents |
