| 摘要: |
| 将5头荷斯坦公牛细管冻精在不同温度，不同时间下进行解冻，对解冻后的精液品质进行检测，结果表明：解冻后顶体正常率，精子活率随解冻温度升高而升高，而畸形率随解冻温度升高而降低（P＜0.01）；高温（37 ℃，75 ℃）下随着解冻时间延长，活率增高（P＜0.05）；低温（5 ℃）,室温（15 ℃）下解冻，活率与解冻时间长短没有明显关系（P＞0.05）；精子顶体正常率，畸形率，存活时间与解冻时间无显著差异。在实践中建议使用75 ℃，2～6 s解冻。 |
| 关键词: 奶牛精液 解冻温度 解冻时间 精液品质 |
| DOI： |
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| 基金项目:陕西省科技攻关项目(4130303) |
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| Effects of different thawing methods on post-thawed motility of cryopreserved cow semen |
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| Abstract: |
| Sixty frozen bovine semen straws (0.25 mL) collected from five Holstein breeding bulls are thawed to determined the effects of different thawing temperature and times on properties of bull semen.The results show that:(1) With the increasing of thawing temperature,the proportion of motile spermatozoa,the proportion of acrosomal integrity,the motible length get improved significantly（P<0.05）.Abnormal spermatozoa rate decreased significantly with the increasing of thawing temperature (P<0.05).(2) The percent of progressive spermatozoa thawed in 37 ℃ or 75 ℃ is higher with the increasing of thawing time,but the percent of progressive spermatozoa is no relation to thawing time.(3) There is no relation among thawing time and the proportion of motile spormatozoa,the proportion of acrosomal integrity,the motible length.It is suggested to thaw at 75 ℃ for 2~6 seconds in practice. |
| Key words: cow semen thawed temperature thawed time semen quality |
