| 摘要: |
| 通过研究不同质量浓度的2,4－D，维生素B1（VB1），水解酪蛋白（CH），腺嘌呤（ADE）和蔗糖等激素及有机物对大蒜体细胞胚胎发生的单一或组合效应，结果表明：0.5 mg/L的2,4－D有利于体胚的发生和发育，梨形胚和棒状胚数目较多；VB1质量浓度提高到0.5～2.0 mg/L可促进体胚高频发生，较高质量浓度的VB1与2,4－D有明显的互作效应；10～30 g/kg的蔗糖能较好地促进大蒜体胚的发生，蔗糖含量超过60g/kg则抑制体胚发生和发育。MS＋2,4－D 0.5 mg/L＋CH 500 mg/L＋ADE 20 mg/L是胚性愈伤组织适宜的继代培养基。 |
| 关键词: 大蒜 体细胞胚胎 诱导因素 |
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| Studies on the induction factors of somatic embryogenesis in garlic (Allium sativum L.) |
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| Abstract: |
| The results obtained from the single or combination effects of different concentration levels of 2,4-Dichlorophenoxy(2,4-D),vitamin B1(VB1),casein hydrolysace(CH),adenosine(ADE)and sucrose on somatic embryogenesis(SE)in garlic indicated that 0.5 mg/L 2,4-D was favorable for SE,and with more pear-shaped and rod-shaped embryos.Increasing the concentration of VB1 to 0.5-2.0 mg/L,the yield of embryos was promoted,and there was obvious interactive effects between higher concentration of VB1 and 2,4-D.The concentration of sucrose 15-30 g/kg was promoted the occurrence of embryos,whereas more than 60 g/kg sucrose was inhibited the occurrence of embryos.MS+2,4-D 0.5 mg/L+CH 500 mg/L+ADE 20 mg/L was the suitable medium for the subculture of embryogenic callus. |
| Key words: garlic somatic embryo induction factors |
