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普通小麦—滨麦—簇毛麦三属杂交后代的细胞遗传学研究
赵继新1, 陈新宏1, 武 军1
西北农林科技大学 农学院
摘要:
对普通小麦-滨麦-簇毛麦三属杂交后代进行了细胞遗传学研究。结果表明:三属杂种杂交结实率较低,平均为14.03%。F1自交结实率极低,平均为0.7%;F1形态兼具普通小麦,滨麦,簇毛麦特性,F2,F3出现严重分离:F1染色体平均构型为2n=12.32I +21.02Ⅱ +0.13Ⅲ +0.09Ⅳ +0.06Ⅴ +0.05Ⅵ,F1出现多多价体,说明来自滨麦的J染色体组和来自族毛麦的V染色体组能形成不紧密的配对:PMC-MI后期I有落后染色体排列在赤道板上,末期I和末期Ⅱ出现大量的二分孢子,四分孢子带微核和畸形孢子现象。这是三属杂种F1育性极低的原因。
关键词:  普通小麦  滨麦  簇毛麦  三属杂种  细胞遗传学
DOI:
分类号:
基金项目:国家自然科学基金项目(39670458);杨凌农业生物技术育种项目(1999-5);西北农林科技大学青年科研专项基金项目
Studies on cytogenetics of the progenies of trigeneric hybridization involving Triticum aestivum,Leymus mollis and Haynaldia villosa
Abstract:
The progenies of trigeneric hybridization involving Triticum,Leymus and Haynaldia were created by crossing between amphidiploids Triti-leymus and Triti-villosa.The cytogenetics and fertility of the progenies were studied.The main results of the study are as followings:(1) The rate of hybrid-fertility was very low (averaged only 14.03%).The self-fertility of F1 plant was also rather low (averaged only 0.8%).(2) The average chromosome configuration of F1 at MI in PMC was 12.32Ⅰ+21.02Ⅱ+0.13Ⅲ+0.09Ⅳ+0.06Ⅴ+0.05Ⅵ,the chromosome number of univalent was less than the theoretical number 14,and the number of multivalents was very high.This indicates that the chromosome set of J (from L.mollis) and V (from Ha.villosa) can untightly pair.(3) At telophase Ⅰin PMC,there were some laggard chromosomes ranked on the equatorial plate,and at telophaseⅠ,telophase Ⅱ,these chromosomes didn't often distribute to two poles equally.It appeared a large number of bispore or tetraspore with micronucleus and abnormal spores.Consequently,a great number of abortive pollen was formed and it made the self fertility of F1 plant rather low.
Key words:  Triticum aestivum  Haynaldia villosa  Leymus mollis  trigeneric hybridization  cytogenetics