| 摘要: |
| 1997－12～2001－08共进行了97批牛卵母胞体外成熟试验，总成熟率为70.75％（3 561/5 033),其中培养液为TCM199＋0.12 g/L丙酮酸钠＋10 mmol/L Hepes 体积分数8％NCS＋10 mg/L FSH +10 mg/L LH +1 mg/L E2(培养液1组）组进行50批试验，成熟率为65.75（1 415/2 152），培养液1组有5批次未添加Hepes盐，其成熟率为75.21％（88/117),另45批次添加Hepes盐，成熟率为65.21％（1 327/2 035），培养液为TCM199＋体积分数8％发情牛血清＋0.12 g/L丙酮酸（培养液2组）组进行47批次试验，成熟率为74.49％（2 146/2 881），培养液2组中有9批次添加了40 mg/L的庆大霉素，其成熟率为72.15％（342/474），另38批次未添加庆大霉素，其成熟率为74.95％（1 804/2 407），试验同时表明，激素来源对卵母细胞成熟率无显著影响。 |
| 关键词: 牛 卵母细胞 体外成熟 |
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| 基金项目:国家“九五”攻关项目(96-C01-03-02-03) |
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| Maturation culture of bovine follicular oocytes in vitro |
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| Abstract: |
| During 1997-12-2001-08,bovine follicular oocytes were cultured in vitro for 97 times,the total percentage of oocytes maturation was 70.75%(3 561/5 033).Among the total expriment,follicular oocytes were cultured in the medium 1 (TCM199+0.12 g/L Pyruvic acid.Na-salt+8% NCS+10 mg/L FSH+1 mg/L LH+1 mg/L β-E2) for 50 times,the maturation rate was 65.75%(1 415/2 152).Among the 50 times,folliclar oocytes were cultured in the medium 1 without Hepes for 5 times,the maturation rate was 75.21% (88/117),the other 45 times follicular oocytes were cultured in the medium 1 with 10 mmol/L Hepes,the maturation rate was 65.21% (1 327/2 035).Follicular oocytes were cultured in the medium 2 (TCM199+0.12 g/L Pyruvic acid.Na-salt+8% OCS) for 47 times,the maturation rate was 74.49%(2 146/2 881).Among the 47 times,follicular oocytes were cultured in the medium 2 with 40 mg/L Gentamycini Sulfas for 9 times,the maturation rate was 72.15%(342/474),the other 38 times follicular oocytes were cultured in the medium 2 without Gentamycini Sulfas,the maturation rate was 74.95%(1 084/2 407).Influence to follicular maturation of hormones was not obvious(75.21% vs 74.95%). |
| Key words: bovine oocytes maturation in vitro |
