| 摘要: |
| 用Wizard DNA Clean-up System试剂盒将与中国野生葡萄抗黑痘病基因相连锁的RAPD遗传标记OPV02-600回收纯化，连接于T-Easy Vector并克隆测序，得到了OPV02-600的全序列，其长度为616 bp。将两端序列设计特异PCR扩增引物作为合成中国野生葡萄抗黑痘病检疫探针的基础，用于检测葡萄抗黑痘病种质和标记辅助育种。 |
| 关键词: 中国野生葡萄 黑痘病 RAPD 克隆 测序 |
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| 基金项目:国家自然科学基金资助项目(39770525);教育部资助年轻优秀教师基金项目;高校博士点基金项目(980701) |
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| Cloning and sequence of RAPD marker linked to anthracnose resistant gene in wild grapes native to China |
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| Abstract: |
| Randomly amplified polymorphic DNA (RAPD) was employed to detect molecular markers linked to anthracnose (Spheceloma ampelinum de Bary) resistant gene in the wild grapes (vitis L.) native to China.RAPD marker OPV02-600 was linked to anthracnose resistant gene using 90-3 cross F1 V.quinquangularis Rehd (shang-24)×V.vinifera (Longyan).OPV02-600 fragment was reclaiment was reclaimed by Wizard DNA Clean-up System from electrophoresis gel and cloned in T-easy Vector,then sequenced from two sides.The DNA fragment OPV02-600 was actually 616 bp.It is suggested that the sequence of RAPD marker OPV02-600 might be used as a basis for synthesizing the specific PCR primers and the probe for detecting grape anthracnose-resistnat in disease resistant breeding and molecular marker assisted selection (MAS). |
| Key words: the wild grapes native to China anthracnose RAPD cloning,sequence |
