| 摘要: |
| 以甘薯丛枝病试管苗病株为材料,用热处理结合茎尖培养法对甘薯丛枝病病株进行了去除植原体的研究.结果表明,0.5 mm以下茎尖培养可以去除植原体,茎尖越小,去除植原体的效果越好,但成苗率降低；0.8 mm以上茎尖培养不能去除植原体,但是成苗率较高。(39±1) ℃高温连续处理2～3周后,剥取茎尖培养可以提高去除植原体的效率,对成苗率影响不大,热处理时间越长,去除植原体效果越好,但被处理的试管苗易死亡。甘薯丛枝病试管苗在(39±1) ℃高温下,以处理3周为宜。 |
| 关键词: 热处理 甘薯丛枝病 植原体 茎尖培养 |
| DOI： |
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| 基金项目:农业部农业生物技术应用研究专项基金(95农-15-04-03) |
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| Obtaining sweet-potato phytoplasma-free plantlets by heat treatment and apical culture |
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| Abstract: |
| Takig the ssweet potatio withch’s-broom plantelts(tissue culture) as the material,the study of obtaining sweet potato witche’s broom-free plantlets by the method of heat treatment and apical culture was carried out.The results showed that apical culture can eliminate the phytoplasma when the size of apical was less than 0.5 mm,however,when the size of apical is more than 0.8 mm,it can’t eliminate the phytoplasma thoroughly.The rate of plantlet formation had close relation with the size of the excised apical.The bigger the apical was,the higher was the rage of plantlets formation.But the rate of phytoplasma-free plantlets had negative relation with the size of the excised apical.The infected sweet potato test-tube plantlets were treated with (39±1) ℃ for 2-3 weeks,then the apicals were excised for culturing in vitro.It was efficient to increase the rate of phytoplasma-free plantlets (it reached 70%) pretreated with heat.At the same time,plantlet formation had no relation with the treatment time,it was only related to the size of excised apical.To sum up,heat treatment and apical culture method is worth while in production of Phytoplasma-free sweet potato plantlets. |
| Key words: heat treatment sweet potato witche's broom phytoplasma apical culture |
